Article ID Journal Published Year Pages File Type
2048464 FEBS Letters 2010 6 Pages PDF
Abstract

Various plant developmental processes involve phytohormones such as cytokinins. Isopentenyltransferase (IPT) reaction is the key rate-limiting step in cytokinin biosynthesis that transfers the isopentenyl (iP) group from dimethylallyl diphosphate to the N6-amino group of adenine. Here, a series of diadenosine polyphosphates (ApnA) were screened as possible substrates of IPT, among which diadenosine tetraphosphate, diadenosine pentaphosphate and diadenosine hexaphosphate showed higher affinity than did the authentic substrates ADP and ATP. In addition, formation of mono-isopentenyl ApnA and di-isopentenyl ApnA was observed. Judging by the existing biosynthetic and hydrolytic systems for ApnA in plants, ApnA and isopentenyl-ApnA may occur in the plant cells, with functional importance.

Related Topics
Life Sciences Agricultural and Biological Sciences Plant Science
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