Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2048468 | FEBS Letters | 2010 | 6 Pages |
Abstract
By combining extracts from strains lacking genes encoding either the maturation enzymes or the large subunits of hydrogenases 1, 2 and 3 we could reconstitute in vitro under strictly anaerobic conditions 10–15% of the hydrogenase activity present in wild type Escherichia coli extracts. Purified, unprocessed Strep-tagged variants of the hydrogenase 2 large subunit, HybC, isolated from either a ΔhybD (encoding the hydrogenase 2-specific protease) mutant or a strain deficient in HypF could also be matured to active, processed enzyme using this system. These studies reveal that minimally one step early on the hydrogenase maturation pathway is oxygen-labile.
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Authors
Basem Soboh, Sara Krüger, Martin Kuhns, Constanze Pinske, Anne Lehmann, R. Gary Sawers,