Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2048625 | FEBS Letters | 2011 | 5 Pages |
The endoplasmic reticulum (ER) is responsible for folding and delivery of secretory proteins to their site of action. One major modification proteins undergo in this organelle is N-glycosylation. Proteins that cannot fold properly will be directed to a process known as endoplasmic reticulum associated degradation (ERAD). Processing of N-glycans generates a signal for ERAD. The lectin Yos9 recognizes the N-glycan signal of misfolded proteins and acts as a gatekeeper for the delivery of these substrates to the cytoplasm for degradation. Presence of Yos9 accelerates degradation of the glycosylated model ERAD substrate CPY∗. Here we show that Yos9 has also a control function in degradation of the unglycosylated ERAD substrate CPY∗0000. It decelerates its degradation rate.
► Non-glycosylated model substrate CPY∗0000 is an ERAD-L substrate. ► The lectin Yos9 retains misfolded CPY∗0000 prior its delivery to the ERAD machinery. ► Yos9 plays a tuning roll in delivering glycosylated and unglycosylated misfolded proteins to ERAD.