Article ID Journal Published Year Pages File Type
2048711 FEBS Letters 2011 5 Pages PDF
Abstract

Activating mutations in different domains of the ABCC8 gene-coded sulfonylurea receptor 1 (SUR1) cause neonatal diabetes. Here we show that a diabetogenic mutation in an unexplored helix preceding the ABC core of SUR1 dramatically increases open probability of (SUR1/Kir6.2)4 channel (KATP) by reciprocally changing rates of its transitions to and from the long-lived, inhibitory ligand-stabilized closed state. This kinetic mechanism attenuates ATP and sulfonylurea inhibition, but not Mg-nucleotide stimulation, of SUR1/Kir6.2. The results suggest a key role for L0 helix in KATP gating and together with previous findings from mutant KATP clarify why many patients with neonatal diabetes require high doses of sulfonylureas.

► Diabetogenic L213R in SUR1 L0 hyperactivates KATP by destabilizing its closed state. ► This kinetic mechanism attenuates KATP inhibition by ATP and sulfonylureas. ► Our analysis reveals a key role for L0 helix in controlling KATP gating.

Related Topics
Life Sciences Agricultural and Biological Sciences Plant Science
Authors
, ,