Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2048874 | FEBS Letters | 2010 | 5 Pages |
Abstract
The genome of lethal animal pathogenic bacterium Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is characterized by the presence of multiple pathogenicity islands (PAIs). Computational methods have been developed to identify PAIs based on the distinguishing G + C levels in some PAI versus non-PAI regions. We observed that PAIs can have a very similar G + C level to that of the host chromosome, which may have led to false negative predictions using these methods. We have applied a novel method of genomic barcodes to identify PAIs. Using this technique, we have successfully identified both known and novel PAIs in the genomes of three strains of EHEC O157:H7.
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Authors
Guoqing Wang, Fengfeng Zhou, Victor Olman, Fan Li, Ying Xu,