Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2048920 | FEBS Letters | 2010 | 4 Pages |
Abstract
Murray Valley encephalitis virus (MVEV) is a member of the flavivirus group, a large family of single stranded RNA viruses, which cause serious disease in all regions of the world. Its genome encodes a large polyprotein which is processed by both host proteinases and a virally encoded serine proteinase, non-structural protein 3 (NS3). NS3, an essential viral enzyme, requires another virally encoded protein cofactor, NS2B, for proteolytic activity. The cloning, expression and biochemical characterisation of a stable MVEV NS2B–NS3 fusion protein is described.
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Authors
Joma Joy, Ng Fui Mee, Wee Liang Kuan, Kwek Zekui Perlyn, Then Siew Wen, Jeffrey Hill,