Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2048952 | FEBS Letters | 2010 | 5 Pages |
Abstract
When a point-mutation in a protein elicits a functional change, it is most common to assign this change to local structural perturbations. Here we show that point-mutations, distant from an essential highly dynamic kinase recognition loop in the response regulator Spo0F, lock this loop in an active conformation. This ‘conformational trapping’ results in functionally hyperactive Spo0F. Consequently, point-mutations are seen to affect functionally critical motions both close to and far from the mutational site.
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Authors
Benjamin G. Bobay, Richele J. Thompson, James A. Hoch, John Cavanagh,