Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2049357 | FEBS Letters | 2010 | 7 Pages |
Abstract
The Zα domain of human ADAR1 (ZαADAR1) preferentially binds Z-DNA rather than B-DNA with high binding affinity. ZαADAR1 binds to the Z-conformation of both non-CG-repeat DNA duplexes and a d(CGCGCG)2 duplex similarly. We performed NMR experiments on complexes between the ZαADAR1 and non-CG-repeat DNA duplexes, d(CACGTG)2 or d(CGTACG)2, with a variety of protein-DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG)2 in the previous study suggests that ZαADAR1 exhibits the sequence preference of d(CGCGCG)2 ≫ d(CACGTG)2 > d(CGTACG)2 through multiple sequence discrimination steps during the B–Z transition.
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Authors
Yeo-Jin Seo, Hee-Chul Ahn, Eun-Hae Lee, Jongchul Bang, Young-Min Kang, Hee-Eun Kim, Yeon-Mi Lee, Kyungmin Kim, Byong-Seok Choi, Joon-Hwa Lee,