| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2049382 | FEBS Letters | 2009 | 7 Pages |
Abstract
Our study aimed to establish the complete structure of the main dihydroxy conjugated triene issued from the lipoxygenation (soybean enzyme) of docosahexaenoic acid, named PDX, an isomer of protectin/neuroprotectin D1 (PD1/NPD1) described by Bazan and Serhan. NMR approaches and other chemical characterization (e.g. GC–MS, HPLC and LC–MS/MS) indicated that PDX is 10(S),17(S)-dihydroxy-docosahexa-4Z,7Z,11E,13Z,15E,19Z-enoic acid. The use of 18O2 and mass spectrometry showed that PDX is a double lipoxygenation product. Its structure differs from PD1, with E,Z,E geometry (PDX) instead of E,E,Z (PD1) and S configuration at carbon 10 instead of R. PDX inhibits human blood platelet aggregation at sub-micromolar concentrations.
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Authors
P. Chen, B. Fenet, S. Michaud, N. Tomczyk, E. Véricel, M. Lagarde, M. Guichardant,