Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2049456 | FEBS Letters | 2007 | 5 Pages |
Abstract
The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C4C5 and C5–C6 bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-RA, but the following millisecond-transition to Meta-RC is blocked. Consistently, no transient proton release was detected. The photoconversion of Agp1-5Zs is apparently arrested in a Meta-RA-like intermediate, since the subsequent syn to anti rotation around the C5–C6 bond is prevented by the lock. The Meta-RA-like photoproduct was characterized by its distinctive CD spectrum suggesting a reorientation of ring D.
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Authors
Sven Seibeck, Berthold Borucki, Harald Otto, Katsuhiko Inomata, Htoi Khawn, Hideki Kinoshita, Norbert Michael, Tilman Lamparter, Maarten P. Heyn,