Article ID Journal Published Year Pages File Type
2049456 FEBS Letters 2007 5 Pages PDF
Abstract

The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C4C5 and C5–C6 bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-RA, but the following millisecond-transition to Meta-RC is blocked. Consistently, no transient proton release was detected. The photoconversion of Agp1-5Zs is apparently arrested in a Meta-RA-like intermediate, since the subsequent syn to anti rotation around the C5–C6 bond is prevented by the lock. The Meta-RA-like photoproduct was characterized by its distinctive CD spectrum suggesting a reorientation of ring D.

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