Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2049466 | FEBS Letters | 2007 | 5 Pages |
Abstract
We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3-ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its activation. This activity involved direct cell–cell contact. A mean force of 20 nN was needed to separate functionalized cells after 5 min of contact. Overall, we showed that it is possible to promote specific cell–cell adhesion by attaching protein ligands at the surface of cells.
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Authors
Aurélie Perier, Christine Gourier, Sylvain Pichard, Julien Husson, Evelyne Lajeunesse, Aurélie Babon, André Menez, Daniel Gillet,