Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2049521 | FEBS Letters | 2010 | 5 Pages |
Abstract
l-Xylulose reductase is part of the eukaryotic pathway for l-arabinose catabolism. A previously identified l-xylulose reductase in Hypocrea jecorina turned out to be not the ‘true’ one since it was not upregulated during growth on l-arabinose and the deletion strain showed no reduced l-xylulose reductase activity but instead lost the d-mannitol dehydrogenase activity [17]. In this communication we identified the ‘true’ l-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on l-arabinose and the deletion results in a strain lacking the NADPH-specific l-xylulose reductase activity and having reduced growth on l-arabinose. The purified enzyme had a Km for l-xylulose of 25 mM and a νmax of 650 U/mg.
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Authors
Dominik Mojzita, Kiira Vuoristo, Outi M. Koivistoinen, Merja Penttilä, Peter Richard,