| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2049721 | FEBS Letters | 2008 | 6 Pages |
Abstract
RBF-2 is a factor comprised of a USF1/2 heterodimer, whose association with a highly conserved upstream element (RBEIII) on the HIV-1 LTR requires a co-factor TFII-I. We have identified specific nucleotides, immediately 3′ of RBEIII that are required for stable association of TFII-I with this region of the LTR. Mutations that inhibit interaction of TFII-I with DNA also prevent stimulation of USF binding to RBEIII, and render the integrated LTR unresponsive to T cell signaling. These results demonstrate an essential role of TFII-I bound at an upstream LTR element for viral replication.
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Authors
Tom Malcolm, Joanna Kam, Pouya Sadeghi Pour, Ivan Sadowski,