Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2049828 | FEBS Letters | 2008 | 5 Pages |
Abstract
Comparative CO photolysis kinetics studies on wild-type and autoregulatory (AR) insert-deletion mutant of rat nNOS holoenzyme were conducted to directly investigate the role of the unique AR insert in the catalytically significant FMN–heme intraprotein electron transfer (IET). Although the amplitude of the IET kinetic traces was decreased two- to three-fold, the AR deletion did not change the rate constant for the calmodulin-controlled IET. This suggests that the rate-limiting conversion of the electron-accepting state to a new electron-donating (output) state does not involve interactions with the AR insert, but that AR may stabilize the output state once it is formed.
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Authors
Changjian Feng, Linda J. Roman, James T. Hazzard, Dipak K. Ghosh, Gordon Tollin, Bettie Sue S. Masters,