Proteomic and genomic analysis of PITX2 interacting and regulating networks

Pituitary homeobox 2 (PITX2) is a homeodomain transcription factor that has a substantial role in cell proliferation and differentiation in various tissues. In this report, we have conducted a systematic study, using proteomic and genomic approaches, to characterize PITX2-interacting proteins and PITX2-regulating genes. We identified four novel PITX2-associated protein partners Y box binding factor-1, heterogeneous ribonucleoprotein K, nucleolin and heterogeneous nuclear ribonucleoprotein U in mass spectrometry analysis. We also found that overexpression of PITX2 upregulated 868 genes (2–25-fold) and downregulated 191 genes (2–15-fold) in DNA microarray analysis. These data provide an insightful perspective for further studying PITX2 function and mechanism of action.Structured summaryMINT-6823857: PITX2 (uniprotkb: Q99697) physically interacts (MI:0218) with hnRNP K (uniprotkb:P61978), YB-1 (uniprotkb:P67809), Nucleolin (uniprotkb:P19338) and hnRNP U (uniprotkb:Q00839) by anti tag coimmunoprecipitation (MI:0007)MINT-6823877: PITX2 (uniprotkb:Q99697) physically interacts (MI:0218) with beta catenin (uniprotkb:P35222), hnRNP U (uniprotkb:Q00839), Nucleolin (uniprotkb:P19338), YB-1 (uniprotkb:P67809) and hnRNP K (uniprotkb:P61978) by anti tag coimmunoprecipitation (MI:0007)MINT-6823902: YB-1 (uniprotkb:P67809) physically interacts (MI:0218) with PITX2 (uniprotkb: Q99697) by anti bait coimmunoprecipitation (MI:0006)