| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2049993 | FEBS Letters | 2009 | 6 Pages |
Abstract
Tobacco (Nicotiana tabacum) Bright Yellow-2 (BY-2) cells can be grown in medium containing indole-3-acetamide (IAM). Based on this finding, the NtAMI1 gene, whose product is functionally equivalent to the AtAMI1 gene of Arabidopsis thaliana and the aux2 gene of Agrobacterium rhizogenes, was isolated from BY-2 cells. Overexpression of the NtAMI1 gene allowed BY-2 cells to proliferate at lower concentrations of IAM, whereas suppression of the NtAMI1 gene by RNA interference (RNAi) caused severe growth inhibition in the medium containing IAM. These results suggest that IAM is incorporated into plant cells and converted to the auxin, indole-3-acetic acid, by NtAMI1.
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Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Keiichirou Nemoto, Masamitsu Hara, Masashi Suzuki, Hikaru Seki, Toshiya Muranaka, Yoshihiro Mano,