Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2050008 | FEBS Letters | 2008 | 6 Pages |
Abstract
Spatial and temporal control of ovine prion protein (Prnp) gene expression was achieved in mice using two transgenes: a Prnp minigene with tet-operator sequences inserted 5′ to exon 1 and a mouse neurofilament genomic clone carrying the chimeric-repressor TRSID cDNA. In bi-transgenic mice, ovine PrPC expression could be reversibly controlled in neuronal cells by doxycycline treatment whereas it remains constant in other cell types. Overall, this model opens opportunities to assess the involvement of cell types in prion diseases and PrP physiological function. It demonstrates the potentiality of the TRSID-silencer to precisely control temporal and spatial gene expression in vivo.
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Authors
Micaela Gallozzi, Vincent Béringue, Pauline Decaunes, Annick Le Dur, Karine Le Roux, Gaëlle Tilly, Sandrine Le Guillou, Laetitia Herzog, Coralie Peyre, Aline Ladroue, Jérôme Chapuis, Marthe Vilotte, Bruno Passet, José Costa, Nathalie Chenais,