Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2050042 | FEBS Letters | 2008 | 4 Pages |
Abstract
Tyrosine nitration results in altered function of selective proteins, including human smooth muscle L-type calcium channel, hCav1.2b. We report here that Cav1.2 is also subject to “denitration”. Cell lysates from activated macrophage-like cell line, RAW264.7 cells, reversed peroxynitrite-induced nitration of the carboxy terminus of Cav1.2 in a 1D gel assay. Tyrosine phosphorylation of the calcium channel by c-src kinase was blocked by nitration but reversed by pretreatment with RAW264.7 cell lysates. These findings indicate that denitration may be a physiological mechanism to restore cellular excitability during inflammation.
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Authors
Minho Kang, Hamid I. Akbarali,