| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2050129 | FEBS Letters | 2008 | 5 Pages |
Abstract
Methylation at the N1 site of adenine leads to the formation of cytotoxic 1-methyladenine (m1A). Since the N1 site of adenine is involved in the hydrogen bonding of T·A and A·T Watson–Crick base pairs, it is expected that the pairing interactions will be disrupted upon 1-methylation. In this study, high-resolution NMR investigations were performed to determine the effect of m1A on double-helical DNA structures. Interestingly, instead of disrupting hydrogen bonding, we found that 1-methylation altered the T·A Watson–Crick base pair to T(anti)·m1A(syn) Hoogsteen base pair, providing insights into the observed differences in AlkB-repair efficiency between dsDNA and ssDNA.
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Authors
Hao Yang, Yingqian Zhan, Dickson Fenn, Lai Man Chi, Sik Lok Lam,