Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2050519 | FEBS Letters | 2007 | 7 Pages |
Abstract
Interleukin 12 receptor β1 (IL-12Rβ1) and β2 (IL-12Rβ2) constitute the functional and high-affinity receptor complex for interleukin 12 (IL-12) and mediate important functions in activated T cells. In this study, we identified cyclin G associated kinase (GAK) as a new IL-12Rβ2-interacting protein using yeast two-hybrid system and confirmed it by coimmunoprecipitation assays. Overexpression of GAK in activated T cells suppresses IL-12 induced IFN-γ production but has no detectable effects on its proliferation, whereas knockdown of GAK by RNA interference (RNAi) increases IFN-γ production. These results suggest that GAK associates with IL-12Rβ2 and may play a role in regulating IL-12 signaling.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Ying Lin, Yu Jie Tang, Hong Liang Zong, Jian Xin Gu, Wei Wen Deng, Chen Wang, Bing Sun,