Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2050556 | FEBS Letters | 2007 | 4 Pages |
Abstract
By analogy to mammals, odorant receptors (ORs) in insects, such as Drosophila melanogaster, have long been thought to belong to the G-protein coupled receptor (GPCR) superfamily. However, recent work has cast doubt on this assumption and has tentatively suggested an inverted topology compared to the canonical Nout − Cin 7 transmembrane (TM) GPCR topology, at least for some Drosophila ORs. Here, we report a detailed topology mapping of the Drosophila OR83b receptor using engineered glycosylation sites as topology markers. Our results are inconsistent with a classical GPCR topology and show that OR83b has an intracellular N-terminus, an extracellular C-terminus, and 7TM helices.
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Authors
Carolina Lundin, Lukas Käll, Scott A. Kreher, Katja Kapp, Erik L. Sonnhammer, John R. Carlson, Gunnar von Heijne, IngMarie Nilsson,