Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2050564 | FEBS Letters | 2007 | 9 Pages |
Abstract
We describe here the cloning and characterization of a novel mouse homeodomain-interacting protein kinase (HIPK)-like gene, Hipk4. Hipk4 is expressed in lung and in white adipose tissue and encodes a 616 amino acid protein that includes a serine/threonine kinase domain. We demonstrate that HIPK4 could phosphorylate human p53 protein at serine 9, both in vitro and in vivo. Among known p53-responsive promoters, activity of the human survivin promoter, which is repressed by p53, was decreased by HIPK4 in p53 functional A549 cells. Human BCL2-associated X protein-promoter activity was not affected. These findings suggest that phosphorylation of p53 at serine 9 is important for p53 mediated transcriptional repression.
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Authors
Shigeki Arai, Akio Matsushita, Kun Du, Ken Yagi, Yasushi Okazaki, Riki Kurokawa,