Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2050645 | FEBS Letters | 2007 | 6 Pages |
Abstract
We identified peroxiredoxin-4 (Prx-4) as a protein interacting with the β isoform of the thromboxane A2 receptor (TPβ) by yeast two-hybrid analysis. Prx-4 co-immunoprecipitated constitutively with TPβ in HEK293 cells. The second and third intracellular loops as well as the C-terminus of TPβ interacted directly with Prx-4. Co-expression of Prx-4 caused a 60% decrease in cell surface expression of TPβ. Prx-4 and TPβ predominantly co-localized in the endoplasmic reticulum. Co-expression of Prx-4 in cells treated with H2O2 targeted TPβ for degradation. We show for the first time an interaction between a receptor involved in oxidative stress and Prx-4, an anti-oxidative enzyme.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Patrick Giguère, Marie-Eve Turcotte, Emilie Hamelin, Audrey Parent, Jessy Brisson, Geneviève Laroche, Pascale Labrecque, Gilles Dupuis, Jean-Luc Parent,