Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2050713 | FEBS Letters | 2008 | 6 Pages |
Abstract
To find out whether we can control plant virus diseases by blocking viral RNA silencing suppressors (RSSs), we developed a strategy to screen inhibitors that block the association of RSSs with siRNAs using a surface plasmon resonance assay. The screened chemicals were tested in competition with RSSs for binding to siRNAs using a mobility shift assay. We then confirmed that tested chemicals actually inhibited the RSS activity in vivo using a protoplast assay which was developed for this purpose. This entire system can be adapted to screening inhibitors of not only plant viruses but also some animal viruses possessing RSSs.
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Agricultural and Biological Sciences
Plant Science
Authors
Hanako Shimura, Takako Fukagawa, Ayano Meguro, Hirokazu Yamada, Mahito Oh-hira, Shinsuke Sano, Chikara Masuta,