Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2050775 | FEBS Letters | 2007 | 6 Pages |
We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B12B12 activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as Coαα-[αα-(7-adenyl)]-Coββ-cyanocobamide or pseudovitamin B12B12. This molecule differs from cobalamin in the αα-ligand, where it has adenine instead of 5,6-dimethylbenzimidazole bound in a αα-glycosidic linkage to C-1 of ribose. L. reuteri is the first lactic acid bacterium in which the production of a cobalamin-like molecule has been identified and the first microorganism reported to produce exclusively pseudo-B12B12.