Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2050890 | FEBS Letters | 2006 | 7 Pages |
Abstract
Evidence showing the existence in the inner compartment of rat-heart mitochondria of AKAP121 and associated PKA is presented. Immunoblotting analysis and trypsin digestion pattern show that 90% or more of mitochondrial C-PKA, R-PKA and AKAP121 is localized in the inner mitochondrial compartment, when prepared both from isolated mitochondria or cardiomyocyte cultures. This localization is verified by measurement of the specific catalytic activity of PKA, radiolabelling of R-PKA by 32P-phosphorylated C-PKA and of AKAP by 32P-phosphorylated R-PKA and electron microscopy of mitochondria exposed to gold-conjugated AKAP121 antibody.
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Authors
Anna Maria Sardanelli, Anna Signorile, Rosanna Nuzzi, Domenico De Rasmo, Zuzana Technikova-Dobrova, Zdenek Drahota, Antonella Occhiello, Alessandra Pica, Sergio Papa,