Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2050945 | FEBS Letters | 2008 | 6 Pages |
Abstract
In higher plants, the PII protein is a nuclear-encoded plastid protein that regulates the activity of a key enzyme of arginine biosynthesis. We have previously observed that Arabidopsis PII mutants are more sensitive to nitrite toxicity. Using intact chloroplasts isolated from Arabidopsis leaves and 15N-labelled nitrite we show that a light-dependent nitrite uptake into chloroplasts is increased in PII knock-out mutants when compared to the wild-type. This leads to a higher incorporation of 15N into ammonium and amino acids in the mutant chloroplasts. However, the uptake differences do not depend on GS/GOGAT activities. Our observations suggest that PII is involved in the regulation of nitrite uptake into higher plant chloroplasts.
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Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Sylvie Ferrario-Méry, Christian Meyer, Michael Hodges,