| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2050976 | FEBS Letters | 2007 | 6 Pages |
Abstract
Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.
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Authors
Futoshi Taura, Supaart Sirikantaramas, Yoshinari Shoyama, Kazuyoshi Yoshikai, Yukihiro Shoyama, Satoshi Morimoto,