Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2050990 | FEBS Letters | 2007 | 8 Pages |
Abstract
We recently established mouse microglial cells persistently infected with mouse-adapted scrapie ME7 (ScMG20/ME7) for in vitro study of prion pathogenesis. Here, we found that ScMG20/ME7 cells were hypersensitive to P2X7 receptor agonists, as demonstrated by sustained Ca2+ influx, membrane pore formation, cell death, and interleukin-1β release. P2X7 mRNA expression was upregulated in these cells, and also in scrapie-infected mice brains. Treatment with pentosan polysulfate eliminated the infectivity and disease-related forms of prion protein from ScMG20/ME7 cell cultures, however, hypersensitivity of P2X7 receptors remained. These results suggest that prion infections may strongly affect the P2X7 receptor system in mouse microglial cells.
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Authors
Takato Takenouchi, Yoshifumi Iwamaru, Morikazu Imamura, Nobuko Kato, Shuei Sugama, Masayo Fujita, Makoto Hashimoto, Mitsuru Sato, Hiroyuki Okada, Takashi Yokoyama, Shirou Mohri, Hiroshi Kitani,