Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2051394 | FEBS Letters | 2007 | 6 Pages |
Abstract
We hypothesized that the ambient air pollution particles (particulate matter; PM) induce cell cycle arrest in alveolar epithelial cells (AEC). Exposure of PM (25 μg/cm2) to AEC induced cells cycle arrest in G1 phase, inhibited DNA synthesis, blocked cell proliferation and caused decrease in cyclin E, A, D1 and Cyclin E- cyclin-dependent kinase (CDK)-2 kinase activity after 4 h. PM induced upregulation of CDK inhibitor, p21 protein and p21 activity in AEC. SiRNAp21 blocked PM-induced downregulation of cyclins and AEC G1 arrest. Accordingly, we provide the evidence that PM induces AEC G1 arrest by altered regulation of G1 cyclins and CDKs.
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Authors
Jingmei Zhang, Andrew J. Ghio, Mingxing Gao, Ke Wei, Glenn D. Rosen, Daya Upadhyay,