| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2051432 | FEBS Letters | 2005 | 7 Pages |
Abstract
Otx3/Dmbx1 is a member of paired class homeodomain transcription factors. In this study, we found that Otx3/Dmbx1 represses the Otx2-mediated transactivation by forming heterodimer with Otx2 on the P3C (TAATCCGATTA) sequence in vitro. The 156 amino acid region (residues 1–156) of Otx3/Dmbx1 is required for its repressor activity, and interacts directly with Otx2. Co-localization of Otx3/Dmbx1 and Otx2 in brain sections was confirmed by in situ hybridization. These data suggest that Otx3/Dmbx1 represses Otx2-mediated transcription in the developing brain. We also identified the consensus binding sequence [TAATCCGATTA and TAATCC(N2-4)TAATCC] of Otx3/Dmbx1.
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Authors
Keita Kimura, Takashi Miki, Tadao Shibasaki, Yan Zhang, Makoto Ogawa, Hiromitsu Saisho, Masaaki Okuno, Toshihiko Iwanaga, Susumu Seino,