Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2051473 | FEBS Letters | 2006 | 6 Pages |
Abstract
We have recently reported a neural variant of microtubule-associated protein 4 with a short pro-rich region (MAP4-SP). Here, we show that the neural MAP4 has reduced microtubule-stabilizing activity, compared to the ubiquitous MAP4 with a long pro-rich region (MAP4-LP), both in vitro and in vivo. Fluorescence recovery after photobleaching analyses revealed that the interaction of MAP4-SP with the microtubules is very rapid, with a half-time of fluorescence recovery of 7 ± 2.36 s, compared to 19.5 ± 3.03 s in case of MAP4-LP. The dynamic interaction of MAP4-SP with microtubules in neural cells may contribute to the dynamic behaviors of extending neurites.
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Authors
Mohammad Rubayet Hasan, Mingyue Jin, Kazuyuki Matsushima, Shigeaki Miyamoto, Susumu Kotani, Hiroyuki Nakagawa,