| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2051547 | FEBS Letters | 2007 | 5 Pages |
Abstract
One way herpes simplex virus type-1 (HSV-1) spreads in vivo is by polykaryocytes formation. Here we demonstrate that polykaryocyte production during HSV-1 spread in cultured human corneal fibroblasts (CF) required heparan sulfate (HS) and more specifically 3-O sulfated HS (3-OS HS). The polykaryocyte formation heavily depended on the expression of HS on target (CF) cells but not on glycoprotein expressing effector cells. Furthermore, we provide the first visual evidence of 3-OS HS and HSV-1 gD colocalization during the membrane fusion process. Taken together our results provide novel insight into the significance of HS in polykaryocyte formation.
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Authors
Vaibhav Tiwari, Gerdy B. ten Dam, Beatrice Y.J.T. Yue, Toin H. van Kuppevelt, Deepak Shukla,