Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2051837 | FEBS Letters | 2006 | 7 Pages |
Abstract
A phosphopantetheinyl transferase (PPTase) gene (pfaE), cloned from the docosahexaenoic acid (DHA)-producing bacterium Moritella marina strain MP-1, has an open reading frame of 861 bp encoding a 287-amino acid protein. When the pfaE gene was expressed with pfaA–D, which are four out of five essential genes for biosynthesis of eicosapentaenoic acid (EPA) derived from Shewanella pneumatophori SCRC-2738 in Escherichia coli, the recombinant produced 12% EPA of total fatty acids. This suggests that pfaE encodes a PPTase required for producing n − 3 polyunsaturated fatty acids, which is probably involved in the synthesis of DHA in M. marina strain MP-1.
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Authors
Yoshitake Orikasa, Takanori Nishida, Akira Hase, Kazuo Watanabe, Naoki Morita, Hidetoshi Okuyama,