Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2052034 | FEBS Letters | 2006 | 5 Pages |
Abstract
Colloidal Force Microscopy was employed to study the viscoelastic and adhesive properties of macrophages upon stimulation with lipopolysaccharide (LPS). Force vs. distance measurements were performed. The adhesion of LPS-stimulated cells (separation force = 37 ± 3 nN) was almost twice as high as that of resting macrophages (16 ± 1 nN). Upon retraction pulling of membrane tethers was observed. Tether lengths and forces at which rupture take place did not depend on stimulation. The reduced Young’s modulus K, a measure of cytoskeleton elasticity, was three times lower than that of the control. The data show that LPS has profound effects on cytomechanical and adhesion properties of macrophages.
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Authors
Stefano Leporatti, Anja Gerth, Guido Köhler, Bernd Kohlstrunk, Sunna Hauschildt, Edwin Donath,