Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2052112 | FEBS Letters | 2006 | 7 Pages |
Abstract
A series of luciferase reporter constructs was prepared from a 1035-bp fragment of mouse genomic DNA flanking the 5′-coding sequence for the SPTLC2 subunit of serine palmitoyltransferase, the initial enzyme of de novo sphingolipid biosynthesis. The full-length DNA fragment promoted strong reporter gene expression in NIH3T3 cells while deletion and site-directed mutagenesis indicated that the proximal 335 bp contain initiator and downstream promoter elements, two proximal GC boxes that appear to stimulate transcription in a cooperative manner, and several additional elements whose activity cannot be accounted for by known factor binding sites. These findings provide insight into the control mechanisms for transcription of mammalian SPTLC2.
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Authors
Stephen C. Linn, Lindsay M. Andras, Hee-Sook Kim, Jia Wei, M. Marek Nagiec, Robert C. Dickson, Alfred H. Merrill Jr.,