Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2052437 | FEBS Letters | 2005 | 6 Pages |
Abstract
This paper explores the relationship between Bax and the mitochondrial permeability transition pore (PTP). Isolated human colon tumor (HCT116) Bax− mitochondria exposed to recombinant Bax exhibited a slow, cyclosporin A-sensitive swelling, but only at [Bax] > 200 nM. The amount of Bax incorporated was much higher than that found in organelles isolated from HCT116 Bax+ staurosporine- or etoposide-treated apoptotic cells, casting doubts on the significance of the putative PT induction for apoptosis. Bax did not influence the electrophysiological properties of an approximately 1 nS channel ascribed to the Ca2+-dependent mitochondrial permeability transition pore. These observations indicate that the PTP is independent of Bax.
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Authors
Silvia Campello, Umberto De Marchi, Ildikò Szabò, Francesco Tombola, Jean-Claude Martinou, Mario Zoratti,