The reaction of neuroglobin with potential redox protein partners cytochrome b5 and cytochrome c

Previously identified, potentially neuroprotective reactions of neuroglobin require the existence of yet unknown redox partners. We show here that the reduction of ferric neuroglobin by cytochrome b5 is relatively slow (k = 6 × 102 M−1 s−1 at pH 7.0) and thus is unlikely to be of physiological significance. In contrast, the reaction between ferrous neuroglobin and ferric cytochrome c is very rapid (k = 2 × 107 M−1 s−1) with an apparent overall equilibrium constant of 1 μM. Based on this data we propose that ferrous neuroglobin may well play a role in preventing apoptosis.