| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2052595 | FEBS Letters | 2006 | 4 Pages |
Abstract
HEN1-dependent methylation of the 3′-terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC-Pro, p21 and p19) inhibit miRNA methylation. These suppressors have distinct effects on different miRNAs. We also show that miRNA∗ is methylated in vivo in a suppressor-sensitive manner, suggesting that the viral proteins interfere with miRNA/miRNA∗ duplexes. p19 and p21 bind both methylated and unmethylated miRNA/miRNA∗ duplexes in vivo. These findings suggest miRNA/miRNA∗ as the in vivo substrates for the HEN1 miRNA methyltransferase and raise intriguing possibilities regarding the cellular location of miRNA methylation.
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Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Bin Yu, Elisabeth J. Chapman, Zhiyong Yang, James C. Carrington, Xuemei Chen,