Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2052603 | FEBS Letters | 2006 | 6 Pages |
Abstract
Nicotianamine is an important metal ligand in plants. Surprisingly, recent genome sequencing revealed that ascomycetes encode proteins with similarity to plant nicotianamine synthases (NAS). By expression in a Zn2+-hypersensitive fission yeast mutant we show for a protein from Neurospora crassa that it indeed possesses NAS activity. Using electrospray-ionization-quadrupole-time-of-flight mass spectrometry we prove the formation of nicotianamine in N. crassa. Transcript level is strongly upregulated under Zn deficiency as shown by real-time PCR. These findings demonstrate that nicotianamine is more widespread in nature than anticipated and provide further evidence for a function of nicotianamine as a cytosolic chelator of Zn2+ ions.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Aleksandra Trampczynska, Christoph Böttcher, Stephan Clemens,