Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2052668 | FEBS Letters | 2006 | 7 Pages |
Abstract
We found that the CutA1 protein, from Pyrococcus horikoshii (PhCutA1), has an extremely high denaturation temperature (Td) of nearly 150 °C, which exceeds the highest record determined by DSC by about 30 °C. To elucidate the mechanism of the ultra-high stability of PhCutA1, we analyzed the crystal structures of CutA1 proteins from three different sources, P. horikoshii, Thermus thermophilus, and Escherichia coli, with different growth temperatures (98, 75, and 37 °C). This analysis revealed that the remarkably increased number of ion pairs in the monomeric structure contributes to the stabilization of the trimeric structure and plays an important role in enhancing the Td, up to 150 °C, for PhCutA1.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Tomoyuki Tanaka, Masahide Sawano, Kyoko Ogasahara, Yasushi Sakaguchi, Bagautdin Bagautdinov, Etsuko Katoh, Chizu Kuroishi, Akeo Shinkai, Shigeyuki Yokoyama, Katsuhide Yutani,