Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2052877 | FEBS Letters | 2006 | 5 Pages |
Abstract
HIV-1 viral protein R (Vpr) is one of the human immunodeficiency virus type 1 encoded proteins that have important roles in viral pathogenesis. However, no clinical drug for AIDS therapy that targets Vpr has been developed. Here, we have established a screening system to isolate Vpr inhibitors using budding yeast cells. We purified a Vpr inhibitory compound from fungal metabolites and identified it as fumagillin, a chemical already known to be a potent inhibitor of angiogenesis. Fumagillin not only reversed the growth inhibitory activity of Vpr in yeast and human cells, but also inhibited Vpr-dependent viral gene expression upon the infection of human macrophages.
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Authors
Nobumoto Watanabe, Yoshifumi Nishihara, Tomoyuki Yamaguchi, Atsushi Koito, Hiroyuki Miyoshi, Hideaki Kakeya, Hiroyuki Osada,