Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2052935 | FEBS Letters | 2006 | 9 Pages |
Abstract
Erythroid Krüppel-like factor (EKLF/KLF1) is an erythroid specific, C2H2 zinc finger transcription factor that is essential for the proper chromatin structure and expression of the adult β-globin gene. Herein, we determine that 26S proteasome inhibitors lead to an accumulation of EKLF protein in murine erythroleukemia (MEL) cells. In addition, EKLF half-life in both MEL cells (<3 h) and fetal liver cells (between 6 and 9 h) is stabilized in the presence of these inhibitors. EKLF is ubiquitinated in vivo, however its modification does not rely on a particular internal lysine. Finally, EKLF contains two PEST sequences within its N-terminus that have no effect on the ability of EKLF to be ubiquitinated but contribute to its destabilization.
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Authors
Karen J. Quadrini, James J. Bieker,