| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2053226 | FEBS Letters | 2005 | 5 Pages |
Abstract
The conformational transition of a plasmid DNA, pGEG.GL3 (12.5 kbp, circular), induced by spermine(4+) was studied through the observation of individual DNA by fluorescence microscopy. We deduced the change in the hydrodynamic radius RH from an analysis of the Brownian motion of single DNA molecules. RH decreases in a continuous manner with an increase in spermine(4+), in contrast to the large discrete on/off change for long linear DNA. Just after the transition to the collapsed state, a small number of DNA molecules tend to form an assembly, which disperses in the bulk solution without precipitation.
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Authors
Yuko T. Sato, Tsutomu Hamada, Koji Kubo, Ayako Yamada, Tsunao Kishida, Osam Mazda, Kenichi Yoshikawa,