Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2053353 | FEBS Letters | 2005 | 4 Pages |
Abstract
A sensor of blue light using FAD (BLUF) protein is a flavin adenine dinucleotide (FAD) based new class blue-light sensory flavoprotein. The BLUF domain of AppA was reconstituted in vitro from apoprotein and flavin adenine dinucleotide, flavin adenine mononucleotide or riboflavin. The light-induced FTIR spectra of the domain reconstituted from various flavins and the 13C-labeled apoprotein showed that identical light-induced structural changes occur in both the flavin chromophore and protein for the signaling state in all of the reconstituted holoproteins. The results showed that an adenosine 5′-dinucleotide moiety is not required for signaling-state formation in a BLUF domain.
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Authors
Shinji Masuda, Koji Hasegawa, Taka-aki Ono,