| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2053354 | FEBS Letters | 2005 | 4 Pages |
Abstract
Gymnemic acids (GA) inhibited rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity. Binding of GA to GAPDH was observed by surface plasmon resonance measurement. Incubation of GAPDH with GA induced a smearing of the GAPDH band in SDS–PAGE. The GA-induced smearing was diminished by prior incubation of GA with γ-cyclodextrin or by GA treatment with NAD. GA treatment did not affect the electrophoretic mobility of glucose-6-phosphate isomerase and dehydrogenase. GA treatment diminished the GAPDH band detected by an antibody to phosphoserine, but did not affect the phosphoserine bands of glucose-6-phosphate isomerase and dehydrogenase. These results indicated that GA specifically induced dephosphorylation of GAPDH.
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Authors
Yusuke Izutani, Takuya Murai, Toshiaki Imoto, Masatake Ohnishi, Masayuki Oda, Sumio Ishijima,