Article ID Journal Published Year Pages File Type
2053405 FEBS Letters 2005 5 Pages PDF
Abstract

In whole-cell recordings on primary cultures of human hepatocytes, we observe the hypertonic activation of a novel type of cation channel with a permeability ratio for Na+:Li+:K+:Cs+:NMDG+ of 1:1.2:1.3:1.2:0.6. With a PCa/PNa of 0.7 the channel is also clearly permeable to Ca++. Most likely, the channel is Cl− impermeable but its activity critically depends on the extracellular Cl− concentration (with the half maximal effect at 88 mmol/l). With a 64% inhibition by amiloride and a complete block by flufenamate and Gd3+ (at 100 μmol/l each), the channel may represent a molecular link between the amiloride-sensitive and insensitive channels reported so far.

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