Article ID Journal Published Year Pages File Type
2056856 Journal of Plant Physiology 2010 5 Pages PDF
Abstract

A novel technique for determining the cis-/trans-stereochemistry of jasmonoyl-isoleucine by coupling its alcoholic derivatives by sodium borohydride with high performance liquid chromatography–tandem mass spectrometry is described. Resolving cis- and trans-stereochemistry of the jasmonates in Achyranthes plants exposed to airborne (exogenous) trans-d2MeJA was demonstrated as an example. This novel application firmly establishes for the first time that trans-d2MeJA is converted exclusively into trans-JA-Ile in Achyranthes leaves, whereas the subsequent de novo biosynthesized JA-Ile possesses cis-stereochemistry. The method is simple, reproducible and could be employed for in vivo cis-/trans-stereochemistry analysis of jasmonates in plants.

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Life Sciences Agricultural and Biological Sciences Agronomy and Crop Science
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