Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2057603 | Journal of Plant Physiology | 2006 | 4 Pages |
Abstract
SummaryA protocol was developed for plant regeneration from encapsulated shoot tips collected from in vitro proliferated shoots of Withania somnifera. The best gel composition was achieved using 3% sodium alginate and 75 mM CaCl2·2H2O. The maximum percentage response (87%) for conversion of encapsulated shoot tips into plantlets was achieved on MS medium supplemented with 0.5 mg/l IBA after 5 weeks of culture. The conversion of encapsulated shoot tips into plantlets also occurred when calcium alginate beads having entrapped propagules were directly sown in autoclaved soilrite moistened with 14‐MS salts.
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Authors
Ajay K. Singh, Rachna Varshney, Manu Sharma, Shyam S. Agarwal, Kailash C. Bansal,