Article ID Journal Published Year Pages File Type
2057603 Journal of Plant Physiology 2006 4 Pages PDF
Abstract

SummaryA protocol was developed for plant regeneration from encapsulated shoot tips collected from in vitro proliferated shoots of Withania somnifera. The best gel composition was achieved using 3% sodium alginate and 75 mM CaCl2·2H2O. The maximum percentage response (87%) for conversion of encapsulated shoot tips into plantlets was achieved on MS medium supplemented with 0.5 mg/l IBA after 5 weeks of culture. The conversion of encapsulated shoot tips into plantlets also occurred when calcium alginate beads having entrapped propagules were directly sown in autoclaved soilrite moistened with 14‐MS salts.

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Life Sciences Agricultural and Biological Sciences Agronomy and Crop Science
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