A simple and efficient transformation system for the edible mushroom Pleurotus eryngii

•The study developed an improved ATMT for generating P. eryngii transformants.•The promoter of Hsp70 from Cordyceps militaris was used tentatively to drive EGFP.•Glufosinate-ammonium was used in genetic transformation system of P. eryngii.•The mycelium hunger-induced was acted as the receptive material.•The transformants were able to produce normal clamp connection.

Although Pleurotus eryngii is a highly-valued mushroom, the tools for undertaking molecular biological research are lacking. We have now developed an improved, more efficient ATMT (Agrobacterium tumefaciens-mediated transformation) system for generating P. eryngii transformants. Successful generation of transformants required that nutrient-limited fungal mycelium be used as the receptor material. The transformants remained genetically stable and produced normal clamp connections in mating tests. The procedures developed here provide an effective and simple means for gene function studies and directed breeding in P. eryngii essential for realizing the full biotechnological potential of this mushroom.